CCCardiol CroatCardiologia CroaticaCardiol. Croat.1848-543X1848-5448Croatian Cardiac SocietyCC 2022 17_9-10_25910.15836/ccar2022.259Extended AbstractExperimental and translational cardiologyThe role of vitamin D and vitamin D binding protein gene polymorphisms in myocardial infarctionhttps://orcid.org/0000-0001-9743-9201RaljevićDamir1*https://orcid.org/0000-0002-7515-6347MiškulinRajko1https://orcid.org/0000-0003-0491-673XKraljević PavelićSandra2https://orcid.org/0000-0003-4473-5431PeršićViktor1Special Hospital for Medical Rehabilitation of the Heart and Lung Diseases and Rheumatism “Thalassotherapia Opatija”, Opatija, CroatiaUniversity of Rijeka, Faculty of Health Studies, Rijeka, CroatiaADDRESS FOR CORRESPONDENCE: Damir Raljević, Thalassotherapia Opatija, Ul. Maršala Tita 188, HR-51410 Opatija, Croatia. / Phone: +385-91-5970-296 / E-mail: damir.raljevic@gmail.com112022179-1025925903112022101120222022Croatian Cardiac SocietyKEYWORDS: myocardial infarctionvitamin D binding proteinsingle nucleotide polymorphism
Background: Cardiovascular diseases are recognized as the leading cause of death in the developed world. Also, the serum level of vitamin D is recognized as one of the risk factors for cardiovascular diseases. Since all active and inactive forms of vitamin D are transported by the vitamin D binding protein (VDBP), it can be assumed that polymorphisms of the VDBP gene that affect its functionality and serum level also affect the serum level of vitamin D and thus may participate in the development of cardiovascular diseases. (1) The aim of this research is to investigate the association of VDBP gene polymorphisms rs4588 and rs7041 with acute myocardial infarction and to investigate the association of these polymorphisms with the serum level of 25-hydroxyvitamin D.
Patients and Methods: This cross-sectional study included 155 subjects with acute myocardial infarction and 105 healthy subjects in the control group. Serum vitamin D level was determined using liquid chromatography tandem mass spectrometry (LC-MS/MS). Allele frequencies at polymorphic sites rs4588 and rs7041 of the VDBP gene were determined using real time polymerase chain reaction (RT-PCR).
Results: A marginally significant association was observed between the VDBP (rs4588) T/T genotype and acute myocardial infarction. Furthermore, we found a significant association between VDBP (rs4588) T/T genotype and the acute anteroseptal myocardial infarction. No association was found between rs7041 VDBP polymorphism and acute myocardial infarction. Although no association of vitamin D serum level with acute myocardial infarction was found, the VDBP (rs4588) G/G genotype was associated with a higher vitamin D serum. Multivariate logistic regression analysis found an association between low vitamin D serum level, VDBP (rs4588) T/T genotype and anteroseptal myocardial infarction.
Conclusions: The results of this study suggest that the VDBP (rs4588) T/T genotype may be associated with acute myocardial infarction of anteroseptal localization. (2) Additional research is needed to further investigate the association of VDBP gene polymorphisms with acute myocardial infarction.
LITERATUREMichosEDMisialekJRSelvinEFolsomARPankowJSPostWS25-hydroxyvitamin D levels, vitamin D binding protein gene polymorphisms and incident coronary heart disease among whites and blacks: The ARIC study.Atherosclerosis. 2015 July;241(1):12–7. 10.1016/j.atherosclerosis.2015.04.80325941991PeršićVRaljevićDMarkova-CarECindrićLMiškulinRŽuvićMVitamin D-binding protein (rs4588) T/T genotype is associated with anteroseptal myocardial infarction in coronary artery disease patients.Ann Transl Med. 2019 August;7(16):374. 10.21037/atm.2019.07.4931555688